Joseph Winlock (February 6, 1826–June 11, 1875) was an American astronomer and mathematician.
He was born in Shelby County, Kentucky, the grandson of General Joseph Winlock (1758–1831). After graduating from Shelby College in Kentucky in 1845, he was appointed professor of mathematics and astronomy at the institution.
From 1852 until 1857 he worked as a computer for the American Ephemeris and Nautical Almanac, and relocated to Cambridge, Massachusetts. He briefly served as head of the department of mathematics at the United States Naval Academy, but returned as superintendent of the Almanac office.
In 1863 he was one of the fifty charter members of the National Academy of Sciences. Three year later in 1866 he became director of the Harvard College Observatory, succeeding George Bond, and making many improvements in the facility. He was also appointed professor of astronomy at Harvard. He remained at the university until his sudden death in 1875, eventually become professor of geodesy.
Much of his astronomical work included measurements with the meridian circle, a catalogue of double stars and stellar photometry investigations. He also led solar eclipse expeditions to Kentucky in 1860 and Spain in 1870.
The crater Winlock on the Moon is named after him.